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It is difficult to know exactly when the shift from gelatine to agar occurred. As often happens for scientific breakthroughs, agar was likely adopted incrementally alongside the use of other growth media. In 1913, for example, the first diagnosis of Serratia marcescens as a human pathogen was made by growing it on agar as well as on potatoes.，这一点在搜狗输入法2026中也有详细论述
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There is a minor artefact in the final file. The stitched .m4a sometimes contains silent padding at the start or end from incomplete chunks at buffer boundaries. A quick ffmpeg pass fixes it cleanly:
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